pe- cy7 anti- mouse cd80 antibody clone: 16- 10a1  (Thermo Fisher)

Journal: The Journal of Immunology Author Choice

Article Title: Cold Mechanical Isolation of Placental Macrophages as a Method to Limit Procedure-Induced Activation of Macrophages

doi: 10.4049/jimmunol.2300379

Figure Lengend Snippet: Abs used for flow cytometry and FACS

Article Snippet: Anti-human CD80 (PE-Cy7) , L307.4 , BD Biosciences , 561135.

Techniques: Flow Cytometry

Journal: The Journal of Immunology Author Choice

Article Title: Cold Mechanical Isolation of Placental Macrophages as a Method to Limit Procedure-Induced Activation of Macrophages

doi: 10.4049/jimmunol.2300379

Figure Lengend Snippet: Gating strategy to define and isolate macrophages by flow cytometry and FACS. (A) Representative images of the gating strategy to sort single, viable macrophages from placental tissue based on the expression of CD45 and CD14. (B) Representative flow cytometric gating strategy to define CD45+ leukocytes. (C) Representative flow cytometric gating strategy to explore the expression of the surface markers CD80, CD86, CD163, and CD206 on CD14+ macrophages.

Article Snippet: Anti-human CD80 (PE-Cy7) , L307.4 , BD Biosciences , 561135.

Techniques: Flow Cytometry, Expressing

Journal: Materials Today Bio

Article Title: Biomimetic liposomes hybrid with erythrocyte membrane modulate dendritic cells to ameliorate systemic lupus erythematosus

doi: 10.1016/j.mtbio.2023.100625

Figure Lengend Snippet: MPA-R-Lipo enhanced cellular uptake efficiency and suppressed the DCs maturation in vitro. (a) BMDCs were cultured for 7 days and then incubated with DiD-labeled Lipo and R-Lipo for 1 ​h, respectively. After that, the cells were stained with actin-specific phalloidin-Alexa 488 and DAPI before being observed under CLSM. Scale bar: 50 ​μm. (b-f) From day 1 of culture, BMDCs were treated with different MPA formulations at the MPA dose of 100 ​ng/mL, then on day 6, BMDCs were stimulated with lipopolysaccharide (LPS) for 18 ​h to induce maturation. (b) Cytotoxicity in CD11c+ cells by propidium iodide stain. Flow cytometric analysis of the CD11c+ BMDCs surface markers (c) CD40 and (d) CD80 expression. ELISA analysis of (e) TNF-α and (f) IFN-γ secretion (n ​= ​3). (ns. No significance).

Article Snippet: Anti-CD11c (PE), anti-CD40 (PerCP-eFluor 710), anti-CD80 (PE/Cy7), and anti-F4/80 (APC-eFluor 780) monoclonal antibodies were bought from eBioscience.

Techniques: In Vitro, Cell Culture, Incubation, Labeling, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Biomimetic liposomes hybrid with erythrocyte membrane modulate dendritic cells to ameliorate systemic lupus erythematosus

doi: 10.1016/j.mtbio.2023.100625

Figure Lengend Snippet: MPA-R-Lipo inhibited the maturation of DCs and alleviated lupus nephritis in MRL/lpr mice. 14-week-old MRL/lpr mice were intravenously given 0.5 ​mg/kg (MPA equivalent) every 4 days and were sacrificed 6 weeks after treatment. (a) Flow cytometric histograms of CD40 and CD80 expression in splenic DCs (CD11c+ cell subset). (b) Flow cytometric histograms of CD40 and CD80 expression in lymphatic DCs. Corresponding quantification of mean fluorescence intensity (MFI) in (c) spleen and (d) lymph nodes. (e) Representative images of kidney H&E (scale bar: 50 ​μm) and IgG and C3 immunostaining (scale bar: 20 ​μm) in glomeruli. (f) BUN and CRE levels in serum. (g) TNF-α and IL-6 levels in serum. (n ​= ​5).

Article Snippet: Anti-CD11c (PE), anti-CD40 (PerCP-eFluor 710), anti-CD80 (PE/Cy7), and anti-F4/80 (APC-eFluor 780) monoclonal antibodies were bought from eBioscience.

Techniques: Expressing, Fluorescence, Immunostaining

Journal: Materials Today Bio

Article Title: Biomimetic liposomes hybrid with erythrocyte membrane modulate dendritic cells to ameliorate systemic lupus erythematosus

doi: 10.1016/j.mtbio.2023.100625

Figure Lengend Snippet: MPA-R-Lipo enhanced cellular uptake efficiency and suppressed the DCs maturation in vitro. (a) BMDCs were cultured for 7 days and then incubated with DiD-labeled Lipo and R-Lipo for 1 ​h, respectively. After that, the cells were stained with actin-specific phalloidin-Alexa 488 and DAPI before being observed under CLSM. Scale bar: 50 ​μm. (b-f) From day 1 of culture, BMDCs were treated with different MPA formulations at the MPA dose of 100 ​ng/mL, then on day 6, BMDCs were stimulated with lipopolysaccharide (LPS) for 18 ​h to induce maturation. (b) Cytotoxicity in CD11c+ cells by propidium iodide stain. Flow cytometric analysis of the CD11c+ BMDCs surface markers (c) CD40 and (d) CD80 expression. ELISA analysis of (e) TNF-α and (f) IFN-γ secretion (n ​= ​3). (ns. No significance).

Article Snippet: Anti-CD11c (PE), anti-CD40 (PerCP-eFluor 710), anti-CD80 (PE/Cy7), and anti-F4/80 (APC-eFluor 780) monoclonal antibodies were bought from eBioscience.

Techniques: In Vitro, Cell Culture, Incubation, Labeling, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Biomimetic liposomes hybrid with erythrocyte membrane modulate dendritic cells to ameliorate systemic lupus erythematosus

doi: 10.1016/j.mtbio.2023.100625

Figure Lengend Snippet: MPA-R-Lipo inhibited the maturation of DCs and alleviated lupus nephritis in MRL/lpr mice. 14-week-old MRL/lpr mice were intravenously given 0.5 ​mg/kg (MPA equivalent) every 4 days and were sacrificed 6 weeks after treatment. (a) Flow cytometric histograms of CD40 and CD80 expression in splenic DCs (CD11c+ cell subset). (b) Flow cytometric histograms of CD40 and CD80 expression in lymphatic DCs. Corresponding quantification of mean fluorescence intensity (MFI) in (c) spleen and (d) lymph nodes. (e) Representative images of kidney H&E (scale bar: 50 ​μm) and IgG and C3 immunostaining (scale bar: 20 ​μm) in glomeruli. (f) BUN and CRE levels in serum. (g) TNF-α and IL-6 levels in serum. (n ​= ​5).

Article Snippet: Anti-CD11c (PE), anti-CD40 (PerCP-eFluor 710), anti-CD80 (PE/Cy7), and anti-F4/80 (APC-eFluor 780) monoclonal antibodies were bought from eBioscience.

Techniques: Expressing, Fluorescence, Immunostaining

Journal: eLife

Article Title: Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain

doi: 10.7554/eLife.80775

Figure Lengend Snippet:

Article Snippet: Antibody , anti-CD80-PE-Cy7 (hamster monoclonal) , eBioscience , Cat. #:25-0801-82 , FC (1:200).

Techniques:

Journal: PLoS ONE

Article Title: Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells

doi: 10.1371/journal.pone.0113388

Figure Lengend Snippet: PBMCs from healthy HLA-DR15+ donors were incubated with MBP (30 µg/ml) in RPMI containing 30% (v/v) normal serum for 18 h. The presentation of MBP85-99 by CD19+ B cells was assessed using biotinylated MK16 and streptavidin-PE. A) Representative dot plot showing a subset of B cells that present MBP58-99 (MK16+) and a subset that do not (MK16−). Expression of various surface markers was evaluated in these subsets. B) The percentages of MK16+ (black bar) or MK16− (white bar) B cells expressing CD27 are shown (N = 4). B-cell expression of the co-stimulatory molecules C) CD86 and D) CD80 is shown as mean fluorescence intensity (MFI) values (N = 5). 7-AAD was used to exclude dead cells. Data are shown as means and SEM.

Article Snippet: For flow cytometric characterisation of B-cell and T-cell subsets, the following fluorochrome-conjugated monoclonal antibodies were used: FITC-anti-human CD3, PE-Cy7-anti-human CD4, PerCP-anti-human CD14, APC-anti-human CD19, FITC-anti-human CD19, PE-anti-human CD27, APC-anti-human CD86 and PE-Cy7-anti-human CD80 (all from BD Biosciences, San José, CA, USA).

Techniques: Incubation, Expressing, Fluorescence

Journal: Nutrients

Article Title: Gene Set Enrichment Analysis Reveals That Fucoidan Induces Type I IFN Pathways in BMDC

doi: 10.3390/nu14112242

Figure Lengend Snippet: Transcription factor pathways with target genes upregulated by fucoidan treatment.

Article Snippet: Cells were stained with the following antibodies for 30 min at 4 °C and analyzed using a flow cytometer (FACS Canto II, BD Biosciences, San Jose, CA, USA) and FlowJo software (Tree Star, Ashland, OR, USA): PerCP-Cy5.5-MHCⅡ (562363, BD Biosciences, San Jose, CA, USA), PE-Cy7-anti-CD80 (25-0801-82, eBioscience San Diego, CA, USA), APC-anti-CD11c (20-0114-U100, Tonbo bioscience, San Diego, CA, USA), APC-Cy7-CD11b (557657, BD Biosciences, San Jose, CA, USA).

Techniques:

Journal: Nutrients

Article Title: Gene Set Enrichment Analysis Reveals That Fucoidan Induces Type I IFN Pathways in BMDC

doi: 10.3390/nu14112242

Figure Lengend Snippet: Leading-edge genes shared by type I IFN signaling pathways in cluster A1.

Article Snippet: Cells were stained with the following antibodies for 30 min at 4 °C and analyzed using a flow cytometer (FACS Canto II, BD Biosciences, San Jose, CA, USA) and FlowJo software (Tree Star, Ashland, OR, USA): PerCP-Cy5.5-MHCⅡ (562363, BD Biosciences, San Jose, CA, USA), PE-Cy7-anti-CD80 (25-0801-82, eBioscience San Diego, CA, USA), APC-anti-CD11c (20-0114-U100, Tonbo bioscience, San Diego, CA, USA), APC-Cy7-CD11b (557657, BD Biosciences, San Jose, CA, USA).

Techniques: